ABSTRACT
Objective: To explore the application value of intraoperative ultrasound (IOUS) combined with neuronavigation in the microsurgery of low-grade glioma (LGG). Methods Totally 34 patients with LGG underwent microsurgery assisted by IOUS combined with neuronavigation. The degree of tumor resection was evaluated by MRI within 72 hours after operation. Occurrence of IOUS related adverse events such as brain contusion, cerebral hemorrhage and complications such as intracranial infection and cerebral hematoma were observed. Results: The detection rate of tumors by IOUS combined with neuronavigation was 100% (34/34) and accuracy rate of localization was 100% (34/34) in the 34 LGG patients. MRI within 72 hours after operation showed total resection in 27 cases (27/34, 79.41%), subtotal resection in 3 cases (3/34, 8.82%), and partial resection in 4 cases (4/34, 11.76%). Intracranial infection occurred in 2 cases which were improved after anti-infection treatment, but no IOUS scan related adverse events occurred. Conclusion: IOUS combined with neuronavigation can accurately locate LGG and achieve maximum extent safely resection of tumors.
ABSTRACT
Objective To investigate the effect of the number of sentinel lymph node (SLN) dissection on prediction of non-sentinel lymph node (nSLN) metastasis in SLN biopsy in patients with early-stage breast cancer. Methods A retrospective analysis of clinical and pathological data of 82 patients with early-stage breast cancer who underwent SLN biopsy and axillary lymph node dissection in the Affiliated Cancer Hospital of Shanxi Medical University from January 2015 to December 2016 was conducted, and the univariate analysis and logistic multivariate regression analysis were performed to analyze the influencing factors of nSLN metastasis. Results Univariate analysis showed that nSLN metastasis was associated with histological grade (χ2 = 10.114, P= 0.006), vascular invasion (χ2 = 12.381, P< 0.01), and the number of positive SLN ≥2 (χ2=13.734, P<0.01), however, it was not related to patient's age, tumor size, SLN metastasis rate, molecular subtype and pathological type (all P>0.05). Multivariate analysis showed that the number of positive SLN≥2 was an independent influencing factor of nSLN metastasis (OR= 4.145, P= 0.015). Conclusions Surgeons need to ensure that the number of SLN dissection is > 2 when the SLN biopsy is performed in patients with breast cancer. When the number of positive SLN is≥2, the risk of nSLN metastasis increases.
ABSTRACT
Objective To investigate the tumor/ non-tumor (T/ NT) ratio during 99 Tcm-hydrazinon-icotinamide(HYNIC)-( poly-( ethylene glycol), PEG) 4-Glu( cyclo ( Arg-Gly-Asp-D-Phe-Lys ( PEG4 ))) 2 ( 99 Tcm-3PRGD2 ) SPECT/ CT imaging and clinical pathological features of breast cancer. Methods Forty-five female patients (age range: 39-76 (53.0±9.5) years) with suspected breast malignant nodules or mas-ses from October 2016 to June 2017 were prospectively enrolled. Patients underwent 99 Tcm-3PRGD2 SPECT/CT imaging before breast puncture and surgery. All subjects had pathological results, and estrogen receptor (ER), progesterone receptor (PR), human epidermal growth factor receptor 2(HER-2), Ki-67 and mi-crovessel density ( MVD) were obtained by immunohistochemistry and fluorescence in situ hybridization(FISH). Two-sample t test and Kruskal-Wallis H test were used to analyze the data. Results Invasive duc-tal carcinoma was pathologically confirmed in 35 of 45 patients. There were 7 patients in stage Ⅰ, 11 pa-tients in stage ⅡA and 17 patients in stage ⅡB. The Luminal A subtype, Luminal B subtype, ERBB2+subtype, Basal-like subtype were found in 6, 9, 9 and 11 patients, respectively. The T/ NT ratio was signifi-cantly higher in the stageⅡB patients than that in stageⅠ+ⅡA patients (4.54±1.46 vs 3.32±1.72, t= -2.24, P<0.05). Patients with ERBB2+ subtype had higher T/ NT ratio compared to patients with Basal-like sub-type: 5.80(3.90, 6.70) vs 2.80(2.20,3.50), H= 11.06, χ2 = 15.31, both P<0.05. Besides, the T/ NT ra-tios in the HER-2 positive group and lymphatic metastasis group were significantly higher than those in the HER-2 negative group and group without lymph node metastasis (t values: -3.99, -2.51, both P<0.05). MVD of HER-2 positive group was higher than that of HER-2 negative group (t= 7.13, P<0.01). Conclu-sion The T/ NT ratio during 99 Tcm-3PRGD2 SPECT/ CT imaging has relations with TNM staging, lymph node infiltration and HER-2 in breast cancer.
ABSTRACT
Objective To evaluate the probability of siblings identification in Identifiler system by using the software of automatic analysis.Methods Using the software of automatic analysis in siblings jdentification.STP genetic typing of 151 pairs of full siblings and 31224 pairs of unrelated individuals from manual simulation were analyzed in 15 STR loci of ldentifiler system.Results Kin probability(W_(FS))of 39.07% full siblings were more than 99.999% while W_(FS) of unrelated individual pairs were 0% .W_(FS) of 60.93% full siblings and 21.3% unrelated individual pairs were all at the range from 99.999% to 1% .W_(FS) of 78.7% unrelated individual pairs 0% full siblings individuals were less than 1% .Therefore,there were notability difference between full siblings and unrelated individual pairs.In addition,testing of 15 STR loci of Identifiler system,it suggested that the pair were siblings when the locus number of the entirely-same is not less than 5 or that of the entirely-different is not more than 1,and that the pair were unrelated individuals when the locus number of the entirely-different is not less than 6 or that of the entirely-8alne is not more than 1.Conclusion The software of automatic analysis and the Identifiler system call be used to siblings identification.